Applied Biology and Biotechnology

 ISSN:  2320 –7825  (Print); eISSN   2320 –7833  (Online)

DNA isolation protocol for different types of seaweeds from seashore areas of Rameshwaram

Babita Kumari

Electrophoretic analysis of hemolymph protein of freshwater crab, Parathelphusa hydrodromous (Hebts.)

B.X. Innocent, B. Victor, and M. Maridass

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Phytochemical studies on Maeraua apetala (Roth.) Jacobs

C. M. Selvaraj, S. Ghanthikumar A. Masillamani and R. Kanthasamy

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Volume No. 1(2)                               March,2013

Table and Contents

Abstract The fresh water crab plays an important role in the study on the immune system of invertebrate and their immunity of bloods. Immune system of invertebrate must rely on non-self-recognition molecules to ensure efficient defense responses against infectious pathogens that continuously threaten their survival. The aim of the present study was carried out the total protein and electrohoretic analysis of hemolymph of freshwater crab, Parathelphusa hydrodromous (Hebts.). The results of the total protein level in P. hydrodromous ranged from 4.0g % to 6.4g % with a mean value of 5.05  0.72g was observed. The conclusion of the results shows hemolymph protein provides a valuable relationship among the various species of crustaceans. 

Keywords: Parathelphusa hydrodromous, Hemolymph, Total protein and Electrophorotic analysis

Abstract In this paper, DNA isolation protocol for development of seaweeds collected from seashore areas of Rameshwaram. The protocol was applied to 10 seaweed species. The protocol yields about 2.5μg of high molecular weight DNA collected from 5mg of dried material of different type of seaweeds, with no RNA. No sign of degradation was observed after agarose gel electrophoresis for both freshly extracted DNA and DNA stored for 18 months at 4°C. The  development of DNA isolation protocol was suitable for genomic library construction (tested for one species), endonuclease restriction, and PCR amplification for all species. Quantification of DNA concentration was performed using UV spectrophotometric measurement of the A260/A280 ratio.

Keywords: CTAB; DNA extraction; nuclei isolation; Seaweed

Abstract

The qualitative and quantitative analyses of Maeraua apetala (Roth.) Jacobs (Capparaceae) was carried out the present study. Powdered leaf materials of chosen plants were extracted with petroleum ether, chloroform, methanol, benzene and distilled water. The preliminary phytochemical analysis of selected plant revealed the presence of steroids, sugar, phenolic groups and amino acids, tannin and absence of triterpene, alkaloids, flavones, saponin, anthroquinone and catachin. Hence, the present study is useful for towards establishing comparative pharmacognostic standards.

Keywords: phytochemical analyses, medicinal plants, Maeraua apetala