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Applied Biology and Biotechnology
ISSN: 2320 –7825 (Print); eISSN 2320 –7833 (Online)
Volume No. 5(4) December,2017
Induction of Somatic Embryogeneis in Janakia arayalpathra Joseph & Chandras. - A RET Medicinal Plant
K. Thangavel, P. Ravichandran, R. Mahesh and R. Uma
Abstract
An efficient and reproducible protocol for the induction of somatic embryos from leaf derived calli of Janakia arayalpathra has been devised. Efficient callus induction was obtained from leaf disc explants in MS medium supplemented with NAA and further proliferation of callus was rapid when BAP and PVP were co-supplemented in the medium. 2 % and 1.2 % of sucrose and agar respectively was found to be ideal for callus induction and establishment. Medium supplemented with NAA + CM + YE influenced differentiation of calli into cell aggregates followed by embryogenic cell mass.
Key Words: Suspension culture, Somatic embryogenesis, Janakia arayalpathra.