Abstract Mosquitoes are the most serious problem in public health, which are responsible for spreading a various number of serious diseases, viz., malaria, filariasis, dengue and Japanese encephalitis. Aim of this present study is evaluation of mosquito larvicidal activity of both plants of Andrographis paniculata (Burm.f.) Wall ex Nees (Acanthaceae) and Aegle marmelos (L.) Correa (Rutaceae) studied for 24h. The both leaf powder materials of A. paniculata (Burm.f.) Wall ex Nees (Acanthaceae) and A. marmelos (L.) were extracted with acetone for 4h in soxhlet apparatus and excess of solvent was removed rotary evaporator under reduced pressure. The crude extracts were dissolved in 1ml acetone and different concentrations of extract prepared and mosquito larvicidal activity was studied for 24 h in laboratory conditions. The results of acetone extract of leaves of A. paniculata (Burm.f.) Wall ex Nees (Acanthaceae) and A. marmelos (L.) Correa (Rutaceae) were showed in the LC50 values of 25.156, and 28.355 respectively active against the mosquito larvae of Culex quinquefasciatus. The conclusion of the present study reported that acetone extracts of both plants of Andrographis paniculata (Burm.f.) Wall ex Nees (Acanthaceae) and Aegle marmelos (L.) Correa (Rutaceae) have a good larvicidal activity. Keywords: Phytochemicals, malaria, medicinal plants, extracts, larvicidal activity, Culex quinquefasciatus