IJBT Journal| Volume No.2(3)

Abstract

The genetic relationships among five Cassia angustifolia accessions from various cultivation areas of Tirunelveli district were analyzed using six RAPD (Randomly Amplified polymorphic DNA) primers. Each primer exhibited 5 – 9 banding patterns for a total of 43 scorable and 19 polymorphic bands (44.19%). The genetic identity and genetic distance ranged from 0.7209 to 0.9302 and 0.0723 to 0.3272 respectively. The observed and effective number of alleles was about 0.5025 and 0.3762. Nei’s (1978) overall gene diversity is 0.2060. The UPGMA dendrogram showed the relationship between five different populations in three clusters.

Keywords: Genetic variability, Cassia angustifolia, Fabaceae, RAPD marker, Polymorphism.

A Study of Seasonal Diversity in the Indoor Mycoflora of Rural Begusarai District of Bihar

Chandan Kumar and Gazala Tabassum

Production and Optimization of Lipase from Bacillus Subtilis

K. Kanimozhi, E.G.Wesely Jebasingh Devairrakam , D.Jegadeeshkumar

Sediment Characteristics along the Ashtamudi Estuarine System

W. Soumya, Tresa Radhakrishnan and S. Radhakrishnan

RAPD – PCR fingerprint analysis of Cassia angustifolia Vahl. in Tirunelveli District of Tamil Nadu

T. Leon Stephan Raj, A. John De Britto, and P. Benjamin Jeya Rathna Kumar

Purification and Immunochemical Characterization of Vicilin storage Protein of Chickpea (Cicer arietinum L.)

Jonnada AV Prasada Rao, Ayub Qadri and K. R. Koundal

Silica gel chromatographic study of phenolic compounds in some species of Indigofera Linn.

Ashok Kumar Roy, Chandan Kumar and Naheed Ahmad

In silico Analysis of Transcriptional Regulators of Pseudomonas aeruginosa and Burkholderia cepacia responsible for Quorum Sensing

S.Jayanthi, B.Sharmila and Palvannan Thayumanavan

Temperature induced chromosomal changes in Indian catfish Heteropneustes fossilis

A. Muthukumaran

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Volume No. 2(3) December, 2011

Table and Contents

Abstract

Fungi are among the most common microbiota found in the houses having unplastered floor in the rural areas of Begusarai district. Fungi of these regions, mostly belongs to the class Ascomycota and Zygomycota. The dust samples were collected in sterilized polythene bag during January 2010 to December 2010 from 4 different spots like Bachhwara, Cheria Bariarpur, Matihani and Sahibpur kamal region of Begusarai district. The mycoflora were isolated using soil dilution plate technique on different culture media, supplemented by suitable antibiotics. Among them nearly 32 mycoflora were identified. Identification and characterization of the mycoflora were made with the help of authentic manuals of fungi. The most common among them viz; Alternaria alternata, Aspergillus fumigatus, Fusarium oxysporum, Mucor hiemalis, Penicillium chrysogenum, Penicillium funiculosum, Penicillium citrinum, Curvularia lunata, Aspergillus niger and Trichoderma viride were isolated, identified and characterized. The seasonal variation and percentage frequency of the mycoflora were statistically analyzed.

Keywords: Microbiota, Ascomycota, Zygomycota, Phylloplane, Colony Forming Units, Moisture, Organic and Inorganic particles

Abstract

In th is st udy, the l ipolytic Bacillus subtilis w as iso lated fr om the oi l m ill w aste by E nrichment techniques. The iso lated c olonies w ere sc reened on O live oi l m edium, colonies w hich pr oduce t he maximum zo ne of t he particular organisms w as used for further op timization s tudies. A mong the 5 Bacillus subtilis isolates a single isolates was the subjected to submerged fermentation medium and the enzyme characteristics were studied with respect to substrate, temperature and pH. The production of lipase is significantly influenced by carbon sources such as Olive oil, Castor oil, Gingelly oil, Palm oil, and S unflower oil at different temperature r ange. The m aximum l ipase ac tivity w as re ached by t he Bacillus `at 37°C and pH 7 where its production reached upto 0.01033 and 0.01066 µg/ml/min. Among the different substrate the maximum activity was observed in Gingelly oil (0.01066 g/ml/min) at pH7 and temperature 37° C. D egradation of o il w astage by the c rude e nzyme ex tract a nd ba cterial suspension wer e com pared. The cru de en zyme e xtract li berate mor e free fa tty a cid (10.95 64 %) compared t o Bacillus isolates (8.42 40%). From the st udy i t w as co ncluded t hat th e commercially important enzyme can be p roduced by submerged fermentation techniques using frequently available edible oil sources it can be used for the biodegradation of oil effluents. Keywords: Bacillus subtilis, Lipase,pH, Temparature, substrate

Abstract

Sediments sampled at monthly intervals from six different regions in the Ashtamudi estuary for a period of one year and analysed for temperature, pH, organic carbon and texture. The temperature of the sediment varied between 27o C and 34o C. The sediment pH was noticeably acidic throughout the study period. The organic carbon content was high in Kandachira kayal where there is hectic coconut husk retting activity. The textural characteristics revealed that the sediments are of sandy nature.

Keywords: Ashtamudi, sediments, backwater, estuary, organic carbon, sediment texture.

Abstract

Chickpea vicilin was isolated and purified by ammonium precipitation, zonal isoelectric precipitation and affinity chromatography on concanavalin-A sephrose. Purified vicilin was resolved on SDS-PAGE into several abundant polypeptides with approximate molecular weight of 66 kDa, 50 kDa, 33 kDa, 27 kDa. The glycosylated nature of vicilin confirms detection of strong signals corresponding to all major band of vicilin on treatment of Con-A. Antisera raised against the purified vicilin shows strong specificity of anti-Cicer vicilin antibodies, when analyzed ELISA, Dot-Blot and Western Blotting. Keywords: Chickpea (Cicer arietinum L.); Storage protein; Vicilin; antivicilin antisera; ELISA

Abstract

The genus Indigofera contains about 700 species, distributed in tropical and subtropical regions of the world. Tropical Africa is its centre of diversity. Phenolic compounds in the leaves of three wild species of Indigofera viz; Indigofera endecaphylla Jacq.,Indigofera enneaphylla Linn. and Indigofera linifolia (L.f.) Retz. were investigated through silica gel chromatographic separation to find out the interrelationship among them. Altogether 20 spots were obtained out of which 9 phenols were identified. Identification and characterization of the phenols were made with the help of Rf value, colour and spectral values of different spots. The identified phenols were- Gallic acid, Caffeic acid, Gentisic acid, Catechol, Coumaric acid, p-OH benzoic acid, Syringic acid, Vanillic acid and Salicyclic acid. The phytochemical data were analyzed with the help of paired affinity (PA), group affinity (GA) and isolation value (IV). The value of Paired affinity (PA) was found highest between Indigofera endecaphylla and Indigofera enneaphylla (66.66%) and lowest between Indigofera endecaphylla and Indigofera linifolia (43.47%). Indigofera linifolia was distantly related to other two species having paired affinity value of 52.17% or less than 50%. Key words: Indigofera, phenols, silica gel chromatography, paired affinity, tropical, phytochemical data, wild species

Abstract

Quorum sensing is the process where bacteria respond to high intracellular concentrations of Autoinducers, which bind to their cognate receptors to regulate the transcription of many virulence genes in many gram negative bacteria including the opportunistic pathogens Pseudomonas aeruginosa and Burkholderia cepacia. These were an important pathogen in patients with cystic fibrosis. Twenty percent of cystic fibrosis patients infected with B. cepacia suffer from cepacia syndrome, a necrotizing pneumonia with fever and occasionally bacteremia. Hence in the present study the 3D structures of the quorum sensing enabled transcriptional activators, LasR from Pseudomonas aeruginosa and CepR from Burkholderia cepacia was generated and the molecular insights was anlaysed by comparative modeling and the their biding sites were analysed. Thus the molecular insight of these modeled structures might serve as a novel point of intervention for de novo design on therapeutic antibiotics.

Key words: Quorum sensing, Comparative Modeling, Pseudomonas aeruginosa, Burkholderia cepacia, Trancriptional Regulators, Autoinducers

Abstract The effect of sudden exposure to temperature on the chromosomal morphology of Indian catfish Heteropneustes fossilis, 15 days after fertilization young ones were exposed to five different higher temperature namely 37,38, 39, and 40°C for a period of four hours. Chromosome spread of the fish from each experiment was prepared and results were documented. The fish when exposed to higher temperatures resulted in chromosomal aberrations such as deletion and translocations. The intensity of chromosomal aberration was more in fish exposed to the highest temperature of 40°C, when compared to other temperatures exposed.

Key words: Heteropneustes fossilis, Chromosome, Cytogenetics, Temperature