Abstract

Nickel induced genotoxic effect on Allium cepa root meristem was evaluated after treating onion root tips with various concentrations of NiCl2 in different time intervals. Mitotic index was higher (9.87 ± 1.96) at initial concentration of 10µg/l in three time intervals (1h,3h and 5h) tested and decreased progressively when NiCl2 concentration and exposure time was increased. Chromosomal gap was found to be very less (1.00) in 30µ/l concentration in all time intervals and found to be increased slightly in higher concentrations. Chromosomal break was found to be highest (36.00) in 100µg/l in all time intervals. Total aberration was found to be increased significantly as concentration increased.

Keywords: genotoxic; Allium cepa; root meristem; Nickel

Abstract

Studies on optimizing of disinfection protocol for Curculigo orchioides have not been reported so far. Here we report the effects of two commonly used surface disinfectants such as HgCl2 (0.1%) and NaOCl (1%) for disinfecting rhizome and leaf explants of C. orchioides at different durations ranging from 1 - 15 min. Treatment with HgCl2 (0.1%) for 15 min was found suitable for rhizome explants whereas leaf explants responded well for HgCl2 (0.1%) treatment for 6 min. Experiment on shoot regeneration shown that 4.4 µM BAP was the optimum concentration for producing healthy shoots. Among the various combinations of auxin and cytokinins tested for root induction, highest percentage of rooting (76.6%) with maximum number of roots (40.4) was recorded when 4.4 µM BAP, 4.6 µM KN and 5.3 µM NAA were combined. The micropropagated plants were similar to the mother plants without any morphological variation. HPLC analysis of the regenerated roots revealed a phytochemical profile similar to that of the mother plant. This protocol can be used to generate elite planting material for large scale cultivation.

 Keywords: Curculigo orchioides, In vitro regeneration, Medicinal plant, High Performance Liquid Chromatography

In vitro regeneration and root induction from Curculigo orchioides Gaertn - A high valued medicinal plant

A. Parameswari, Rahul R. Nair S. Thilaga and D. Ganesh

Rapid biosynthesis of silver nanoparticles using fern leaflet extract and evaluation of their antibacterial activity

A.R. Nalwade, M. N. Badhe, C. B. Pawale,  and S. B Hinge

Analysis of Nickel Induced Genotoxicity in Root Meristem of Allium cepa

A. Ganesan and N. Panneerselvam 

In Vitro and Micropropagation for Conservation of Rare and Threatened medicinal Plant Ceropegia species - A Review

K. Kalimuthu and R. Prabakaran

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Volume   No.4(2)                                 August,2013

Abstract

India has a rich biological diversity due to its varied climatic, altitudinal variations and ecological habitats. There have been increasing rates of threats of depletion to these biological resources due to immense biotic and abiotic stresses. Indiscriminate collection of plants for their medicinal, ornamental, perfumery uses, etc. and habitat loss and degradation are potential causes of threats. Conventionally, are methods allow conservation to occur with ongoing natural evolutionary processes, ex situ conservation via in vitro propagation also acts as a viable alternative for increase and conservation of populations of existing bioresources in the wild and to meet the commercial requirements. A review highlighting various in vitro protocols developed for rare and threatened plant called Ceropegia species. India has been done to highlight the significance of ex situ conservation in cases where regeneration through conventional methods is difficult to undertake and species are left with low population in the wild.

Keywords: Ceropegia, in vitro propagation, micropropagation, conservation, threatened