International Journal of  Biological Technology
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Gayathri Global Academic & Scientific Publisher (GGASP),India
ISSN: 0976-4313 Print

Volume   No.8(1)                             April,2017

Table and Contents

 

Enzymatic activities of midgut of two Earthworm species of Perionyx excavatus and Lampito mauritii

 

V. Mariappan, and K.Kaleeswari

 

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The effects of ethanolic extract of Indigofera longirecemosa leaves incorporated with basal diet fed on growth and disease challenges on Vibrio parahaemolyticus infection of the giant freshwater prawn Macrobrachium rosenbergii post larvae

 

G. Raju and M. Maridass

 

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Abstract:

The aim of the present study to analyzing the enzymatic activity of amylase, cellulase, protease, acid phosphatase and alkaline phosphatase in mid gut content of Lampito mauritii and Perionyx excavates were assessed. The experimental method was carried out for a period of 60 days of culture period. The collection of earthworms into the troughs i.e., at the initial day (1st day) and after an interval of middle (30th day) and final (60th day) the samples from mid gut of earthworms were collected and analyzed for the enzymes activity. The results of the present study, the enzyme an activity in mid gut content of Lampito mauritii and Perionyx excavates was increased with increasing composting periods and all the values obtained in the present experiments were showed significant changes. Interestingly, protease activity showed a negative correlation with all others in the case of Perionyx excavates. Keywords: Earthworm, Lampito mauritii, Perionyx excavates, midgut, enzymatic activities

Abstract : The present study was to evaluate the basal diet supplemented with 1%, 2%, 3% and 4% extract of Indigofera longirecemosa leaves fed on the growth performance, disease challenge and histopathology analysis of post larvae of Macrobrachium rosenbergii for 60 days. The experimental methods designed for the prawns were divided into control, infected prawn, and treated groups fed with control diet and infected prawns treated with different concentration of 1%, 2%, 3% and 4% I. longirecemosa leaves extract. All experimental groups were diseases challenged using with intramuscularly injected with 30μl of Vibrio parahaemolyticus (1×107 cfu/ml) on prawn of post larvae. The disease challenge study was observed by 30day period, the five prawns were randomly selected from each tank for each experimental and control group to haemolymph collection. The cumulative mortality and survival rate were assessed every day. The histological analysis of hepatopancreas examined the end of the experimental periods. The prawns were fed on basal diet supplemented with 1%, 2%, 3% and 4% I. longirecemosa leaves showed good growth performance was observed. The specific growth rate of the prawn was observed the highest in the 4 % ethnolic extract incorporated feed treatment compared to all others treatment groups. Prawns fed the 4% diet had highly significantly higher (p< 0.05) than the control group. Histopathological examination of the present study observed that hepatopancreas of control prawns exhibited in the wellorganized structure of glandular tubular normally seen in the control. V. parahaemolyticus. 4% ethanolic extract had significantly more E-cells within their hepatopancreatic tubules compared to other treatments while after 56 days, the hepatopancreatic tubules of prawns in the 4% extract treatment were more closely arranged with significantly more R- and E-cells. In conclusion, this study suggested that the formulation of the experimental diet composition of basal diets supplemented with ethanolic extracts of I. longirecemosa were effective as growth promoters and treatment bacterial infection of M. rosenbergii