A New Record of tetraploid cytotype of Gymnocarpium dryopteris (L.) Newman (Dryopteridaceae - Pteridophyta) from Uttar Pradesh, India

S.Dominic Rajkumar, Shashank Kumar Singh, Shobhit Kumar Srivastava, Ravi Pratap Gautam

Gymnocarpium dryopteris is globally widespread in tropical and warm temperate region. Gymnocarpium is from Greek origin (gymnos means ‘naked’ and karpos means ‘fruit’) a reference to the lack of indusia and Dryopteris is also a Greek term (drys means ‘oak’ and Pteris means ‘fern’) so commonly known as oak fern tree belonging to the fern family Dryopteridaceae.

Callus induction in the leaf segments of Jasminum angustifolium
 
Priya Joy, D. Patric Raja and S. Iruthaya Kalai Selvam 

Abstract Jasminum angustifolium commonly called Wild jasmine in English and Kattumullai in Tamil and belongs to the family Oleaceae. The explants were finally rinsed four - five times with sterile distilled water and inoculated on MS (Murashige and Skoog, 1962) supplemented with various concentrations of growth regulators. The interaction of the auxin (NAA) with the cytokinin (BAP) in the concentrations tested were not effective to obtain callus in the leaf explants of J. angustifolium. However, in the presence of 0.5, 1.0, 1.5, 2.0 and 2.5 mg L-1 NAA and in the absence of BAP, root emission was observed in the explants. This also happened when they were inoculated in the presence of 2.0 and 3.0 mg L-1 NAA with 1.0 mg L-1 BAP. The highest callus formation in the leaf explants of J. angustifolium was observed with the isolated addition of 2, 4- D to the culture medium. There was no callus formation in the absence of this growth regulator. The interaction of the auxins NAA and 2,4- D with the cytokinin BAP, in the tested concentrations, are not enough for callus production in the leaf explants of J. angustifolium. The presence of 2,4-D is essential for callus induction in the leaf explants of this species. When used by itself in the culture medium, TDZ is not effective in inducing callogenesis in the leaf explants of J. angustifolium. Maximum production of callus in the leaf explants of J. angustifolium is obtained when they are cultured on MS medium, supplemented with 2.5 or 3.0 mg L-1 2,4-D. Keywords: Jasminum angustifolium, tissue culture, micropropagation, plant growth regulators, callus.

 Volume No. 2(1)                                                                             MARCH,2013