Fern Journal
Pteridological Research
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Peer - Reviewed Journal
Volume No. 1(1) June,2012
Identification of developmental stages of Actiniopteris radiata Swartz) Link.,
M. Maridass, G. Raju, R. Mahesh, K. Muthuchelian and K.Dharmar
Table and Contents
Abstract
In this present investigation, we have studied the effects of both MS medium and heavy metals concentration on spore germination and developmental gametophyte stages of Pteris tripartita Sw. Among four different concentration of MS medium, the spore germination rate was significantly increased in half strength MS medium (80%) followed by full strength MS medium (70%) and significant gametophyte growth was observed in ½ strength MS medium in 45 days. The effects of heavy metals such as ZnSo4, CuSo4, HgCl2, CdCl2, Na3AsO4. 7H2O on spore germination were also studied. The heavy metals culture medium swiftly caused the spore germination percentage and its protonemal cells. The in vitro spore germination, protonemal growth appraisal could be used to evaluate the fern tolerance on heavy metals and also its toxicity on environmental pollution.
Keywords: spore germination, heavy metals, Pteris tripartita, protonemal cells
Abstract
The phytochemical analysis of Adiantum latifolium leaves and their crude extract assessed for antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The crude extract of A. latifolium leaves were analyzed by bioautographic method and phytochemicals analysed through thin layer chromatography (TLC) method. The results were indicated that the antibacterial zones of A. latifolium extract on TLC plate were positive to phenolic/flavonoid compounds. Therefore, this result suggested that A. latifolium possessed anti-bacterial activity and the extract would be a potential source for antibacterial agents. Keywords: Adiantum latifolium; Adiantum; TLC; antibacterial activity
Abstract
The fern gametophyte is a small plant that exists as an intermediate in the fern life cycle, between the germination of a spore and the mature sporophyte. The gametophytic generation of Actiniopteris radiata was observed in spore morphology, spore germination and also development stages of the gametophytes. The spores of A. radiata were trilete in shape, 50x50µm size and characteristics of apogamous type gametophytes observed.
Keywords: Pteridophytes; Actiniopteris radiate; gametophytes;spores;