The present study aims the chemical composition of the entire plant of Indigofera trita L. carried out by analyzing sample collected from Yercaud hills in the Eastern Ghats of Tamil Nadu, for chemical composition. The result of analysis shows that the presence of various chemical constituents like carbohydrates, glycosides, alkaloids, tannins, saponins, phenolic compounds, phytosterols and flavonoids were present. The significant amount of phyto-constituent of this plant may open the new window to pharmacological research in different dimensions. Keywords: Indigofera trita, Chemical composition, Characterization, TLC
Abstract In the present study, tuber extract of Eulophia epidendraea responses on nutritional, challenges on bacterial disease and pathological analysis of common carp fish, Cyprinus carpio (L.). Feeding experimental analysis of basal diet with 5% of tuber extract of Eulophia epidendraea responses on nutrition, bacterial disease challenges and pathological analysis of common carp fish, C. carpio conducted for 42 days. Results of control fed with 5% tuber extract E. epidendraea responses on good peripheral haemogram of diseased carps, C. carpio. The significant antibacterial response in the experimental fish was observed on 14th and 28th days. The curative effect of the tuber extract of E. epidendraea peaked at 14th and 28th days after the feeding regimen, suggesting the development of immunotolerance to the decreased fish which could able to regain better health after feeding the tuber extract. Keywords: Eulophia epidendraea; tuber; Common carp; fish, Cyprinus carpio Nutritional; Bacterial; Disease; Challenges ; Pathological analysis, macrophages
Abstract A new, simple, sensitive, selective and precise high-performance liquid chromatography (HPLC) method for analysis of Gallic acid in raw material & related formulations was developed and validated. Raw materials (Terminalia chebula, Terminalia bellerica and Emblica officinalis) & finished product (Triphala churna) were taken for the study. The stationary phase was inertsil C18 column. The mobile phase consisting of acetonitrile (HPLC grade) and potassium dihydrogen phosphate buffer in a gradient flow were used. The column was equilibrated with the mobile phase (flow rate 1.0 ml/min) & elution was monitored at 270 nm. Keywords: Gallic acid, HPTLC, method validation, Terminalia chebula, Emblica officinalis
